 |
In order to produce a DNA microarray dedicated to investigate a wide range of biological effects related to the inflammatory processes, we have identified through an in silico approach a set of 5000 genes in Human, mouse and Rat that share the property to be inducible by the main immunomodulators in mammals.
Such approach leads to get a nearly exhaustive list of cytokine inducible genes which is called CIGsearch™ (for Cytokines-Induced Genes) in comparison to genes that are identified either by intensive bibliographic searches in a field of interest (in this case, the selection is limited by the key words used for the query) or by experimentations (the selection is restricted to particular experimental conditions).
The gene selection was based on the identification within the whole human genome of specific DNA motifs that were defined experimentally to control transcriptional expression in response to cytokines. These motifs were previously identified by our team and belong to three groups. Search for all three regulatory motifs leads us to identify thousands of genes in human, mouse and rat genomes that were used as probes for a dedicated microarray, CIGsearch™ allowing to quantify expression of 5000 genes responding to the main mediators of inflammation.
| 
